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KSHV-induced ligand mediated activation of PDGF receptor-alpha drives Kaposi's sarcomagenesis

Fig 2

Phosphorylation of PDGFRA in mECK36 and KS tumors is associated with the presence of its PDGF ligands and localize to areas of KSHV infection.

(A) PDGFA, PDGFB and GAPDH determined in 3 different samples of Normal Skin and mECK36 tumors from 3 different mice by immunoblotting. (B) Immunohistochemical staining of mouse KS-like mECK36 tumors for PDGFA, PDGFB, LANA, and phospho-PDGFRA. Representative image of the FFPEs obtained from the mECK36 tumorigenesis experiment N = 5.(C) Immunohistochemical staining of human KS biopsies for PDGFA, PDGFB, LANA, and phospho-PDGFRA. (D) IFA of mECK36 tumors for KSHV LANA (red) and phospho-PDGFRA (green). Cell nuclei were counterstained with DAPI (blue). White arrows indicate cells that co-stain for LANA and p-PDGFRA.

Fig 2

doi: https://doi.org/10.1371/journal.ppat.1007175.g002