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Innate immune sensor LGP2 is cleaved by the Leader protease of foot-and-mouth disease virus

Fig 2

Expression of catalytically active FMDV Lbpro induces LGP2 cleavage.

HEK293 cells were co-transfected with a plasmid encoding hLGP2-Myc-DDK and (A) LbWT or EV and lysed at the indicated times after transfection, (B) EV or increasing amounts of a plasmid encoding LbWT (0, 0.2, 2, 20, 200 or 2000 ng) and lysed 24 h later. (C) HEK293 cells were co-transfected with plasmids encoding hLGP2-Myc-DDK and LbWT in the presence of zVAD or MG132 caspase and proteasome inhibitors, respectively. In control cells, apoptosis was induced with puromycin. Cells were lysed 24 h later. (D) HEK293 cells were co-transfected with plasmids encoding DDK-poLGP2 and LbWT, LbC51A, EV or water (-). Control cells were co-transfected with plasmids encoding hLGP2-Myc-DDK and LbWT. (E) HEK293 cells were co-transfected with plasmids encoding DDK-poLGP2 and LbWT, LbC51A or EV and lysed at the indicated times after transfection. (F) HEK293 cells were co-transfected with plasmids encoding hLGP2-Myc-DDK or DDK-poLGP2 and LbWT and lysed 24 h later. (G) HEK293 cells were co-transfected with plasmids encoding DDK-poLGP2-Myc and LbWT or LbC51A and lysed 24 h later. (H) Porcine SK6 cells were mock-transfected (-) or co-transfected with plasmids encoding DDK-poLGP2 and LbWT, LbC51A or EV and lysed 24 h later. Cell lysates were analyzed by western blot for the indicated proteins using the specified antibodies. The N- and C-terminal cleavage products of LGP2 are indicated with arrows. A putative C-terminal cleavage product of poLGP2 harboring the Myc tag is indicated by an asterisk.

Fig 2

doi: https://doi.org/10.1371/journal.ppat.1007135.g002