Defects in intracellular trafficking of fungal cell wall synthases lead to aberrant host immune recognition
(A) Schematic of the Mar1 protein domains. DUF, domain of unknown function; TM, transmembrane. (B) The mar1ΔT-DNA insertional mutant and a mar1Δ full deletion mutant are dry on pH 8 and sensitive to 1.5 M NaCl. Serial dilutions of the indicated strains were spotted onto YPD, YPD with 150 mM HEPES at pH 8, or YPD with 1.5 M NaCl. (C) The mar1Δ strain shares distinct and overlapping sensitivities to cell wall stressors with other cell wall integrity pathway mutants. Strains were serially diluted and spotted onto YPD with the addition of the indicated cell wall stressors or at the indicated temperatures. Melanin was assessed by the production of brown pigment on Niger Seed agar at 30°C. CFW, calcofluor white; SDS, sodium dodecyl sulfate.