Methyl-CpG-binding (SmMBD2/3) and chromobox (SmCBX) proteins are required for neoblast proliferation and oviposition in the parasitic blood fluke Schistosoma mansoni
(A) Representative NIH-3T3 fibroblast cells transfected with full-length SmMBD2/3-FLAG and RFP-FLAG constructs are illustrated. Scale bar = 10 μm. DAPI represents transfected cells visualised with a 405 nm blue diode laser; SmMBD2/3 represents SmMBD2/3-FLAG transfected cells visualised with a 488 nm argon laser; RFP represents RFP-FLAG transfected cells visualised with a 561 nm diode-pumped, solid state laser; Merge represents cellular superimposition of DAPI/SmMBD2/3-FLAG or DAPI/RFP signals. (B) Relative SmMBD2/3-FLAG and RFP-FLAG nuclear (Nuc) vs cytoplasmic (Cyto) fluorescence was quantified from LSCM images collected from a total of 40 transfected cells/construct. SmMBD2/3-FLAG was found to be significantly enriched within nuclear compared to cytoplasmic compartments (p < 0.05). SmMBD2/3-FLAG nuclear localisation was also found to be significantly greater than RFP-FLAG nuclear localisation in the transfected cells (p < 0.01).