Advertisement

< Back to Article

Neisseria gonorrhoeae employs two protein inhibitors to evade killing by human lysozyme

Fig 6

Ng_1063 and Ng_1981 are important for Gc survival from physiologically relevant sources of human lysozyme.

A. WT, Δ1981, Δ1981::1981+ complement, Δ1981Δ1063, and Δ1981Δ1063::1063+ complement Gc were exposed to pooled human tears (diluted to 0.01X) that had been pretreated with recombinant 1981 (r1981) or mock-treated for 3 hr. Gc survival was determined as in Fig 2B. *p < 0.05; two tailed t-test n = 4–9 biological replicates. B. WT, Δ1981, Δ1981::1981+ complement, Δ1981Δ1063, and Δ1981Δ1063::1063+ complement Gc were exposed to pooled human saliva (diluted to 0.05) that had been pretreated with r1981 or mock-treated for 3 hr. Gc survival was determined as in Fig 2B. *p ≤ 0.05; two tailed t-test, n = 4–9 biological replicates. C. Adherent IL-8-treated primary human neutrophils were exposed to WT, Δ1063, Δ1981, Δ1981::1981+ complement, and Δ1981Δ1063 Gc at an MOI of 1. At indicated time points, CFU were enumerated from neutrophil lysates. Percent Gc survival was determined by dividing CFU/mL at each time point by CFU/mL at 0 min. *p ≤0.05 for Δ1981 compared to WT or Δ1981::1981+ complement and †p ≤0.05 for Δ1981Δ1063 compared to WT; two tailed t-test, n = 3–17 independent experiments. All values are represented as the mean ± SEM.

Fig 6

doi: https://doi.org/10.1371/journal.ppat.1007080.g006