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Neisseria gonorrhoeae employs two protein inhibitors to evade killing by human lysozyme

Fig 5

Ng_1063 and Ng_1981 are important for Gc survival from human lysozyme.

A. WT, Δ1981, Δ1981::1981+ complement, Δ1981Δ1063, and Δ1981Δ1063::1063+ complement Gc were exposed to human lysozyme for 3 hr. Gc survival was determined as in Fig 2B. *p < 0.05; two tailed t-test, n = 3–31 biological replicates. B. WT, Δ1981, and Δ1981Δ1063 Gc were exposed to 1,000 μg/mL human lysozyme (HL), and CFU/mL was determined over time. CFU were enumerated at the indicated times. *p < 0.05 comparing Δ1981 or Δ1981Δ1063 to WT, and p < 0.05 comparing Δ1981Δ1063 to Δ1981; two tailed t-test, n = 3–6 biological replicates. C. WT, Δ1981Δ1063, and Δ1981Δ1063::1063+ complement Gc were exposed for 3 hr to 10 μg/mL human lysozyme (HL) with or without boiling for 1 hr to eliminate enzymatic activity. Gc survival was determined as in Fig 2B. *p < 0.05; two tailed t-test, n = 5–9 biological replicates. D. Δ1981, Δ1981Δ1063, Δ1981Δ1063::1063(WT)-FLAG complement, Δ1981Δ1063::1063(S83A)-FLAG complement, and Δ1981Δ1063::1063(K103A)-FLAG complement Gc were exposed to the indicated concentrations of human lysozyme for 3 hr. Gc survival was determined as in Fig 2B. NS, not significant. *p < 0.05; two tailed t-test, n = 7–10 biological replicates. All values are represented as the mean ± SEM.

Fig 5

doi: https://doi.org/10.1371/journal.ppat.1007080.g005