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Metabolic reprogramming of Kaposi’s sarcoma associated herpes virus infected B-cells in hypoxia

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Metabolic characterization of KSHV negative/positive cells growing in normoxia and CoCl2 or 1% O2 induced hypoxia.

(A&B) Western blot analysis of HIF1α for the confirmation of hypoxia induction in BJAB and BJAB-KSHV cells growing in the presence of CoCl2 or1% O2. (C) Time dependent glucose uptake estimation in culture media from BJAB/BJAB-KSHV cells growing in normoxia or CoCl2 induced hypoxia. (D) Time dependent lactate released estimation in culture media of BJAB/BJAB-KSHV cells growing in normoxia or CoCl2 induced hypoxia. (E) Time dependent glucose uptake estimation in culture media from BJAB/BJAB-KSHV cells growing in normoxia or 1% O2 induced hypoxia. (F) Time dependent lactate released estimation in culture media of BJAB/BJAB-KSHV cells growing in normoxia or 1% O2 induced hypoxia. (C), (D), (E) and (F) represent mean of three independent experiments. Asterisk (*) indicates differences which are statistically significant (BJ = BJAB, BK = BJAB-KSHV, * p≤0.05, **≤0.01). (G). LANA immune-staining showing KSHV infection of PBMC. The bar diagram represents KSHV infected cells as measured microscopically for LANA immune-staining. (H) Western blot analysis of HIF1α for the confirmation of hypoxia induction in control and KSHV infected PBMCs growing in the presence of CoCl2. (I) Glucose uptake estimation in control and KSHV infected PBMCs at 48 hours post infection and grown under normoxia or CoCl2 /1% O2 induced hypoxia. (J) Estimation of lactate released by control and KSHV infected PBMCs at 48 hours post infection and grown under normoxia or CoCl2 /1% O2 induced hypoxia. The mean values from individual experiments were used to plot graph and the error bars were calculated based on differences from mean value (* p≤0.05, **≤0.01).

Fig 1

doi: https://doi.org/10.1371/journal.ppat.1007062.g001