Peroxisomes support human herpesvirus 8 latency by stabilizing the viral oncogenic protein vFLIP via the MAVS-TRAF complex
(A-B) Immunoblots of extracts from 293T cells transfected with the indicated TRAFs and vFLIP (A) and with WT and catalytic mutant of HA-TRAF3 or Flag-TRAF6 along with vFLIP (B). Arrow indicates a band of unknown identity, which was used as a loading control. (C) Immunoblotting and co-immunoprecipitation (co-IP) analyses of extracts from WT and MAVS KO 293T cells transfected with 500 ng V5-vFLIP and the indicated amounts of Flag-TRAF6 vector. IgG-Lc indicates light chain of immunoglobulin. (D) Diagram of V5-vFLIP showing the position of a TRAF-interacting motif (TIM) and its mutation (TIMX), in which P93 and Q95 were substituted with leucine (L) and arginine (R), respectively. (E) Co-IP assay with extracts from 293T cells transfected with V5-vFLIP WT and TIMX mutant along with the indicated TRAF plasmids. (F) Immunoblots of extracts from 293T cells transfected with V5-vFLIP WT and TIMX mutant in the presence and absence of Flag-MAVS.