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Peroxisomes support human herpesvirus 8 latency by stabilizing the viral oncogenic protein vFLIP via the MAVS-TRAF complex

Fig 4

MAVS stabilizes vFLIP via the CARD region and TRAF-interacting motifs.

(A) Diagram of Flag-MAVS showing the position of its caspase activation and recruitment domain (CARD), proline-rich domain (PD), and transmembrane (TM) regions. (B) Immunoblots of extracts from 293T cells transfected with Flag-MAVS full-length (FL) and deletion mutants (ΔCARD, ΔPD, and ΔTM) together with non-tag vFLIP or V5-vFLIP. (C) Diagram of MAVS mutations of the CARD and TRAF-interacting motifs (TIMs). (D) Immunoblots of extracts from 293T cells transfected with Flag-MAVS WT and point mutants together with V5-vFLIP. (E) Immunoblots of extracts from BCBL-1 MAVS KO (1A4) cells lentivirally transduced with WT and mutants (2ED and QN2ED) of MAVS (MAVSRg1) resistant to the MAVS gRNA1.

Fig 4

doi: https://doi.org/10.1371/journal.ppat.1007058.g004