Peroxisomes support human herpesvirus 8 latency by stabilizing the viral oncogenic protein vFLIP via the MAVS-TRAF complex
(A-B) Growth curve of wild-type (WT) and MAVS knockout (KO) BCBL-1 cell lines (A) and BJAB cells infected or uninfected with HHV-8 BAC16 virus (B). Cell viability was assessed by determining ATP levels using CellTiter-Glo for 4 days. MAVS and GFP expression were determined on day 0 by immunoblot as shown next to each graph. Data are presented as mean ± SD of triplicate samples. Probability (p) values at day 4 are depicted; * p<0.05 and ** p<0.01. (C-D) Flow cytometry analyses of annexin V-FITC and 7-AAD in WT and MAVS KO BCBL-1 (1A4) cells seeded at 5x104 and 2x105 cells/ml and incubated for 2 days (C) and seeded at 5x104 cells/ml and treated with 20 nM rapamycin (Rapa) for 2 days (D). The percentage of total cells in each quadrant is shown.