Advertisement

< Back to Article

Assembly-hub function of ER-localized SNARE proteins in biogenesis of tombusvirus replication compartment

Fig 4

Repression of the expression of either Ufe1p or Use1p SNARE proteins inhibits peroxisome biogenesis in yeast.

(A) Confocal laser microscopy analysis of subcellular distribution of BFP-SKL peroxisomal luminar marker in GALS::UFE1 yeast cells grown under repressive conditions (in glucose media). Note that the cytosolic distribution of BFP-SKL and the lack of co-localization of with GFP-p33 demonstrating inhibition of peroxisome biogenesis. (B) Confocal laser microscopy analysis of subcellular distribution of BFP-SKL in GALS::UFE1 yeast cells grown in galactose media (i.e., inducing condition). (C-D) The effect of expression of the dominant-negative Ufe1p mutant (Ufe1ΔTM) on subcellular distribution of BFP-SKL in wt yeast strain. RFP-Ufe1ΔTM was expressed 6 hours after induction of BFP-SKL and GFP-p33 (panel C) or simultaneous expression of the three proteins from plasmids (panel D). (E) WT yeast control not expressing Ufe1ΔTM. (F) Confocal laser microscopy analysis of subcellular distribution of BFP-SKL peroxisomal luminar marker in GAL1::USE1 yeast cells grown under repressive conditions (in glucose media). Note that the cytosolic distribution of BFP-SKL and the lack of co-localization of with GFP-p33 demonstrating inhibition of peroxisome biogenesis. (G) Confocal laser microscopy analysis of subcellular distribution of BFP-SKL in GAL1::USE1 yeast cells grown under inducing conditions.

Fig 4

doi: https://doi.org/10.1371/journal.ppat.1007028.g004