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p38b and JAK-STAT signaling protect against Invertebrate iridescent virus 6 infection in Drosophila

Fig 3

JAK-STAT signaling is required for IIV-6-induced turandot expression and survival from virus infection A) S2* cells were transfected with dsRNA targeting hopscotch, domeless, or Stat92E, and 48 hours later were infected with IIV-6 for 24 hours. RNA was then isolated and TotA induction was quantified by qRT-PCR. Data shown are from three biologically independent assays. Two non-overlapping dsRNAs were used to target each gene. Error bars indicate standard deviation and black bars indicate mean. Statistical analysis was performed comparing control dsRNA (GFP or mCherry) transfected cells to target gene knockdowns by two-way ANOVA with corrections for multiple comparisons using the Holm-Sidak method (**** p <0.0001). A.U., Arbitrary Units. B) Kaplan-Meier curves showing survival of Stat92ERNAi expressing (UAS-Stat92ERNAi (VDRC)x tubulin-Gal4) flies (green lines) or control (w1118 x tubulin-Gal4) flies (black lines) following infection with IIV-6 (solid lines) or injection with PBS (dotted lines). C) Kaplan-Meier curves showing survival of Stat92ERNAi expressing (UAS-Stat92ERNAi (TRiP-1)x c564-Gal4) flies (green lines) or control (w1118) flies (black lines) following infection with IIV-6 (solid lines) or injection with PBS (dotted lines). Survival assays utilized at least 50 animals per treatment in each trial and statistical significance was determined by Log-rank (Mantel-Cox) test, comparing IIV-6 infected RNAi lines to IIV-6 infected control animals, or PBS-injected RNAi lines to PBS-injected controls. ****p <0.0001.

Fig 3

doi: https://doi.org/10.1371/journal.ppat.1007020.g003