p38b and JAK-STAT signaling protect against Invertebrate iridescent virus 6 infection in Drosophila
Turandot genes are expressed upon IIV-6 Infection A) Heatmap of mRNA levels for selected immune-related genes following IIV-6 infection of adult w1118 flies for 12, 24 and 48 hours assayed in duplicate by NanoString nCounter. RNA was isolated from PBS-injected flies at the same time points. Each data point is a biologically independent sample. A’) Detailed comparison of mRNA levels for Tot A or Tot M from nCounter data. B) Fold induction of all eight Tots from w1118 flies infected with IIV-6, relative to PBS injected controls, at 6, 12, or 24 hours, quantified by qRT-PCR. n = 3, error bars represent SEM and statistical significance determined by Multiple t-tests with correction for multiple comparisons using the Holm-Sidak method. By this analysis, all Turandots were significantly induced with p values between 0.05 and 0.0003 at all time-points with the exception of TotF (ns) and TotE, which was undetectable (nd). C) S2* cells were infected with IIV-6 and TotA expression was assayed by qRT-PCR at the indicated time points. Significance was determined by two-way ANOVA and Sidak’s multiple comparisons test, comparing the infected sample to its time-matched uninfected control. * p < 0.05; *** p < 0.001; and **** p < 0.0001 Error bars indicate standard deviation and black bars indicate the mean. A.U., Arbitrary Units.