Advertisement

< Back to Article

Varicella zoster virus productively infects human natural killer cells and manipulates phenotype

Fig 2

IL-2 stimulation of NK cells, CD3+CD56+ lymphocytes, and T cells enhances VZV infection.

(A) Healthy human donor PBMCs were infected with VZV by cell-associated infection with or without IL-2 (200 U/ml) for 2 days, then analysed by flow cytometry. Plots show surface VZV gE:gI expression from one representative donor and graphs show frequency of live gE:gI+ NK cells (CD3CD56+) (top panels), CD3+CD56+ lymphocytes (middle panels), and T cells (CD3+CD56) (bottom panels). Symbols represent individual donors consistent across lymphocyte populations, and bars indicate mean (n = 8). ***p < 0.001, ****p < 0.0001 (two-tailed paired t test). (B & C) Healthy human donor CD56+-selected lymphocytes were infected with VZV by cell-associated infection with or without IL-2 (200 U/ml) for 2 days, then analysed by flow cytometry. Plots show surface gE:gI expression from one representative donor and graphs show frequency of live gE:gI+ NK cells (B) or CD3+CD56+ lymphocytes (C). Symbols represent individual donors, consistent across (B & C) (n = 7). *p < 0.05 (two-tailed Wilcoxon matched-pairs signed rank test).

Fig 2

doi: https://doi.org/10.1371/journal.ppat.1006999.g002