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Fab-based inhibitors reveal ubiquitin independent functions for HIV Vif neutralization of APOBEC3 restriction factors

Fig 4

3C9 is a non-competitive inhibitor of Vif-mediated A3F-CTD ubiquitination.

(A) Fluorescence polarization binding assay for VCBC binding to fluorescently labeled A3F-CTD in the absence (black) or presence (red) of 3C9. (B) In vitro ubiquitination of Myc-tagged A3F-CTD in the presence of increasing amounts of Flag-tagged 3C9. A3F-CTD and 3C9 were visualized by WB using anti-Myc and anti-Flag, respectively.

Fig 4