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Fab-based inhibitors reveal ubiquitin independent functions for HIV Vif neutralization of APOBEC3 restriction factors

Fig 2

3C9 and 1D1 disrupt Vif-mediated A3 ubiquitination in vitro.

Ubiquitination of Myc-tagged (A-C) A3F-CTD or (D-F) A3G in the absence or presence of increasing amounts of 3C9, 3F12, and 1D1 (0.1 μM, 0.5 μM, and 1μM). NL4-3 Vif E3 ligase was used for ubiquitination assays and A3 proteins were visualized by WB with anti-Myc.

Fig 2