The potent effect of mycolactone on lipid membranes
Fig 5
Interaction of mycolactone with mixed monolayers in the presence or absence of cholesterol.
Adsorption kinetics (π-t) curves of mycolactone on monolayers composed of (A) mixture 1 (39% POPC, 33% SM, 9% POPE, 19% Chol) or (B) mixture 2 (48% POPC, 41% SM, 11% POPE) at 20°C (solid line) or 25°C (dashed line). Mycolactone was injected (4.45 μL) into the PBS subphase (pH 7.4) beneath the monolayer compressed at an initial surface pressure πi of 30 mN/m after a relaxation time of one hour (arrows). Surface area was kept constant during the run. The final concentration of mycolactone was 60 nM. Each measurement was performed at least three times for each condition, and a representative curve is presented here.