Bacteria and bacterial envelope components enhance mammalian reovirus thermostability
A488-labeled (A) T1L and (B) T3D reovirus were not incubated (NI) or incubated with PBS, 50 μg/ml detoxified LPS (dLPS), or 50 μg/ml PG for 2 h at room temperature. HeLa cells were incubated with 10 μg/ml JAM-A specific antibody for 1 h at 4°C prior to adsorption with reovirus at an MOI of 5×103 particles/cell for 1 h at room temperature. Cell surface reovirus (MFI) was assessed by flow cytometry. *, P < 0.0005 compared to PBS incubated virus by one-way ANOVA with Dunnett’s multiple-comparison test.