Bacteria and bacterial envelope components enhance mammalian reovirus thermostability
(A) Reovirus T1L and T3D were not incubated, incubated with PBS or increasing concentrations of mannan, chitin, mucin, lipoteichoic acid (Lipo Acid), or PG for 2 h at room temperature. (B) Reovirus T1L and T3D were not incubated, incubated with PBS or increasing concentrations of BSA, milk, sucrose, polystyrene beads (PS Beads), or PG for 2 h at room temperature. PS beads concentration is shown as the ratio of virions to beads. (A, B) HeLa cells were adsorbed with reovirus at an MOI of 5×103 particles/cell, incubated for 18 h, and scored for infectivity by indirect immunofluorescence. Results are expressed as box and whisker plots of percent infectivity (normalized to no incubation) for quadruplicate independent experiments. *, P ≤ 0.0005 in comparison to PBS by one-way ANOVA with Dunnett’s multiple-comparison test.