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Bacteria and bacterial envelope components enhance mammalian reovirus thermostability

Fig 5

Association of reovirus with bacteria enhances attachment to eukaryotic cells.

(A) A488-labeled T1L and T3D reovirus were incubated with mCherry-expressing B. subtilis for 2 h at room temperature and imaged by confocal microscopy. (B) A488-labeled T1L and T3D reovirus were not incubated or incubated with PBS, 20 μg/ml PG, or mCherry-expressing B. subtilis for 2 h at room temperature and adsorbed to HeLa cells for 1 h. Cells were stained with CellTracker Blue and imaged by confocal microscopy. Scale bar, 10 μm.

Fig 5

doi: https://doi.org/10.1371/journal.ppat.1006768.g005