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Bacteria and bacterial envelope components enhance mammalian reovirus thermostability

Fig 4

Lipopolysaccharide and peptidoglycan protect reovirus from loss of attachment and infectivity.

(A, B) Reovirus T1L and T3D were not incubated, incubated with PBS, detoxified LPS (dLPS), LPS, or PG for 2 h at room temperature. (C) Reovirus T1L and T3D were not incubated or incubated with PBS for 2 h at room temperature. 50 μg/ml LPS or 50 μg/ml PG were added at indicated times during 2 h incubation. (A) HeLa cells were adsorbed with A633-labeled reovirus at an MOI of 5×103 particles/cell and assessed for reovirus attachment by flowcytometry. Results are expressed as box and whisker plots of cell surface reovirus as MFI for quadruplicate independent experiments. (B, C) HeLa cells were adsorbed with reovirus at an MOI of 5×103 particles/cell, incubated for 18 h, and scored for infectivity by indirect immunofluorescence. Results are expressed as box and whisker plots of percent infectivity (normalized to no incubation) for quadruplicate independent experiments. *, P < 0.0005 in comparison to PBS by one-way ANOVA with Dunnett’s multiple-comparison test.

Fig 4

doi: https://doi.org/10.1371/journal.ppat.1006768.g004