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Bacteria and bacterial envelope components enhance mammalian reovirus thermostability

Fig 2

Gram positive and Gram negative bacteria positively affect reovirus thermostability.

(A) Reovirus T1L and T3D were not incubated, incubated with PBS or increasing concentrations of B. subtilis or E. coli for 2 h at room temperature. HeLa cells were adsorbed with reovirus at an MOI of 5×103 particles/cell and assessed for infectivity in HeLa cells 18 hpi by indirect immunofluorescence. Results are expressed as box and whisker plots of percent infectivity (normalized to no incubation) for quadruplicate independent experiments. (B) A488-labeled reovirus T1L and T3D were not incubated, incubated with PBS or B. subtilis or E. coli for 2 h at room temperature and 5×103 particles/cell were assessed for attachment to HeLa cells by flowcytometry. Results are expressed as box and whisker plots of cell surface reovirus as mean fluorescence intensity (MFI) for quadruplicate independent experiments. *, P < 0.0005 in comparison to PBS by one-way ANOVA with Dunnett’s multiple-comparison test.

Fig 2

doi: https://doi.org/10.1371/journal.ppat.1006768.g002