Stably expressed APOBEC3H forms a barrier for cross-species transmission of simian immunodeficiency virus of chimpanzee to humans
Fig 4
SIVcpz is inhibited by hA3H hapII by both deaminase dependent and independent mechanisms.
(a) SIVcpzPttMB897Δvif reporter constructs were co-transfected with hA3G, hA3H hapII or hA3H hapII E56A expression plasmids, PTR600 empty vector was used as control (vector). Two days post-transfection, normalized amounts of viruses were used to infect 293T cells. The nanoluciferase (relative light units-RLU) was measured two days post-infection. The expression of A3s was detected by anti-HA antibody, tubulin served as loading control. (b) The viral supernatants from (a) were treated with DNase I (20 units for 1 ml viral supernatant) and used to infect 293T cells. 12 h post-infection, the cellular DNA was isolated. A 700-bp fragment of SIVcpz was amplified by PCR, cloned and sequenced.