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Strains of bacterial species induce a greatly varied acute adaptive immune response: The contribution of the accessory genome

Fig 3

The effect of phage KO on adaptive immune response.

A. and C. CFSE labeled PBMC from 10 donors were stimulated with strains Newman or TB4 (Newman KO of its 4 phages) as in Fig 1A, stained and analyzed by FACS for % proliferation (CFSE dilution) of live CD3+CD4+ cells (A left), % IFNγ expression by proliferating live CD3+CD4+ cells (A right), % proliferation of B cell (live CD3-CD19+, C left), or % IgG expression by live B cells (C right). B. Example of FACS plot of one of the donors in Figure A. D. Intracellular staining for IFNγ, IL17A expression in proliferating live CD3+CD4+ cells following stimulation with either Newman or TB4. E. As in D, but staining for transcription factor Tbet and RORγt. F. and G. are as in A and C left, respectively, but after stimulation with wild type Streptococcus pyogenes M1 strain SF370 or its complete phage KO. H. Intracellular staining for IFNγ, IL17A expression in proliferating live CD3+CD4+ cells following stimulation with either wild type Streptococcus pyogenes M1 strain SF370 or its complete phage KO (CEM1ΔΦ). I. The MANOVA test for difference between bivariate means of % T cell proliferation and % B cell proliferation cells following stimulation with either TB4 or Streptococcus pyogenes M1 (SF370) complete phage KO (CEM1ΔΦ) strains.

Fig 3

doi: https://doi.org/10.1371/journal.ppat.1006726.g003