LipL21 lipoprotein binding to peptidoglycan enables Leptospira interrogans to escape NOD1 and NOD2 recognition
(A) Survival curves (left panel) and weight variation (right panel) of C57BL6/J (WT) and NOD1 and NOD2 deficient (NOD1/2DKO) mice after intraperitoneal infection with 4 different doses (from 5.108 to 4.106 leptospires/mouse) of MFLum1, a bioluminescent strain of L. interrogans serovar Manilae L495 (weekly passage 14). Survival curves and weight loss were established within the 5 days following the infection, corresponding to the acute phase of the disease. Percentage of weight loss is represented as the mean ± SEM of individual mice compared to their initial weights before infection at day 0 (D0). For each dose, n = 5 WT and n = 5 NOD1/2 DKO mice. (B) Bacterial loads in WT and NOD1/2DKO mice IP infected with a sub-lethal dose of 107 bioluminescent L. interrogans (MFLum1). Bacterial loads were measured by qPCR in blood at 8, 24, 48 and 72 hours post infection, and in urine at 5 and 7 days post infection, and by live imaging (IVIS), 1 month post infection. The graphs represent a compilation of 3 independent experiments with n = 3 to 6 mice for the blood panel, 1 experiment for the urine panel and 1 experiment representative of 3 with n = 4 mice for the IVIS panel. (A),(B). Statistics are not indicated as no significant differences were found at any time points between WT and NOD1/2DKO mice.