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Spatiotemporal dynamics of HSV genome nuclear entry and compaction state transitions using bioorthogonal chemistry and super-resolution microscopy

Fig 4

Quantitation of EdC labelled genomes in individual HSV-1EdC particles.

Equivalent samples of HSV-1EdC or HSV-1[17] at 1x108 pfu/ml were adsorbed onto glass coverslips prior to detection by cycloaddition and immunofluorescence for VP5. Panels I and IV show the merged channel images for each virus (scale bar 10 μm). The inset in panel I shows a magnified section. Panels II and V show only the green channel (genome detection) for each virus. Panels III and VI show the colour-coded outline overlay produced by the ImageJ plugin used for particle analysis (described in materials and methods); yellow indicates particles containing both VP5 capsid protein and EdC genome signal; red indicates VP5+ve particles lacking detectable EdC; green indicates particles with detectable EdC but no VP5. The data for approximately 700 particles are quantified in the right panels for each virus.

Fig 4

doi: https://doi.org/10.1371/journal.ppat.1006721.g004