Spatiotemporal dynamics of HSV genome nuclear entry and compaction state transitions using bioorthogonal chemistry and super-resolution microscopy
Higher magnification images from experiment as described for Fig 2. Cells were processed by cycloaddition for EdC incorporation together with immunofluorescence for ICP8 and counterstained by DAPI staining for total DNA (DAPI). Individual channels are shown in grey scale and the merged images in colour. Images were acquired with an x63 objective (scale bar 10 μm). Patterns of localisation are as discussed in the text.