Unraveling the key to the resistance of canids to prion diseases
10% brain homogenates from transgenic N158D PrP mouse lines (Tg402 and Tg403) were seeded with the different mouse prion strains (RML, 301C and 22L) at the indicated dilutions and subjected to one single 48h PMCA round. All samples [non-PMCA amplified (NA) and amplified samples (A)] were digested with 85 μg/ml of protease-K (PK) and were analyzed by Western blot using SAF-83 (1:400) monoclonal antibody. No mouse prion strains were able to propagate in any of the transgenic N158D PrP mouse brain homogenates. C(Mo): undigested mouse brain homogenate. Mw: Molecular weight.