A conserved fungal glycosyltransferase facilitates pathogenesis of plants by enabling hyphal growth on solid surfaces
(A) Global clustering analysis of fungal RNAseq data from growth of wild type and ΔZtGT2 mutants in liquid culture, and at 48h after leaf inoculation. Key to data point labelling- WT_plant_R1 indicates wild type fungus on plant surface 1st replication; MT_cul_R2 indicates ΔZtGT2 mutant in YPD culture broth 2nd replicate sample. (B) Rapid arrest of growth on wheat leaf surfaces is indicated by dramatically reduced expression of many genes encoding ribosomal protein in ΔZtGT2. (C) Many genes induced and repressed by the WT fungus following leaf inoculation retain the same dynamics in ΔZtGT2 mutants. (D) Many classes of early expressed genes encoding secreted proteins are up-regulated to equivalent levels in both the WT and ΔZtGT2 mutant strains indicating surface sensing is still retained. All data is expressed relative to expression levels detected in the equivalent fungal liquid culture. (NLP = necrosis and ethylene inducing protein).