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A conserved fungal glycosyltransferase facilitates pathogenesis of plants by enabling hyphal growth on solid surfaces

Fig 2

All mutant phenotypes of 23–170 result from inactivation of a gene encoding a putative type 2 glycosyltransferase (ZtGT2).

(A) The gene model structure identified through TAIL-PCR analysis of the T-DNA insertion site in mutant 23–170. (B) RNAseq raw read mapping confirmed the predicted gene structure and highlights the position of the left border T-DNA insertion. (C) Functional annotation of the tagged gene highlighting the position of the T-DNA left border relative to the predicted type 2 glycosyltransferase catalytic domain. (D) The predicted amino acid sequence of ZtGT2 highlighting the protein region truncated by T-DNA insertion in brown font. The underlined region indicates the peptide sequence chosen for antibody generation (E) Complementation of the 23–170 mutant strain with the wild-type ZtGT2 gene (23–170::GT2comp) restores hyphal growth on solid surfaces and virulence on plants. Independent targeted deletion of ZtGT2 in the wild-type fungus (ΔZtGT2-19) results in the same aberrant hyphal growth and loss of virulence phenotypes shown for 23–170.

Fig 2