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A conserved fungal glycosyltransferase facilitates pathogenesis of plants by enabling hyphal growth on solid surfaces

Fig 1

Characterisation of a non-pathogenic T-DNA mutant of Z. tritici (23–170) unable to extend hyphae on solid surfaces.

(A-D) Typical growth characteristics of wild type (WT) Z. tritici on rich nutrient agar at 16°C (A); rich agar at 25°C (B); sterile liquid water (C) and solid water agar (D). (E-H) display the comparable growth morphologies of mutant 23–170. Note the severely impaired hyphal growth from the colony on solid water agar (H). (I-K) micrographs displaying hyphal morphology of wild type Z. tritici on solid water agar. (L-N) comparable micrographs displaying morphology of short, sinusoidal hyphae formed by 23–170. (O) SEM analysis of wild type fungal inoculated wheat leaf surfaces at 48 hpi. Arrows highlight surface growing hyphae. (P) SEM of 23–170 mutant cells at 48hpi on wheat leaf surfaces. Arrows highlight un-germinated fungal spores. (Q) Wheat leaf disease symptoms photographed 14 days after inoculation with wild type fungus. (R) Comparable levels of leaf disease caused by the 23–170 mutant at 14 dpi.

Fig 1