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HIF-1α is a key regulator in potentiating suppressor activity and limiting the microbicidal capacity of MDSC-like cells during visceral leishmaniasis

Fig 8

CD11c-specific HIF-1α-knockout mice produce more monocyte’s progenitors and display enhanced output of inflammatory monocytes in the bone marrow.

Hifflox/flox-Cd11c-Cre+ and Cre- mice were infected with L. donovani and sacrificed at various time point of infection. (A) Gating strategy to identify GMPs in the bone marrow of L.donovani infected mice. (B) Frequency (left graph) and absolute numbers (right graph) of GMPs in the bone marrow of mice over the course of infection. (C-E) Frequency (upper graph) and absolute numbers (lower graph) of granulocytes (C), Ly6Chi monocytes(D), Ly6Clo/int monocytes (E) in the bone marrow of infected mice. (F-G) Percentage of CCR2+ (F) and CXCR4+ (G) Ly6Chi monocytes in the bone marrow. (H) Percentage of Sca-1+ Ly6Chi monocytes in the bone marrow. (I) Percentage of MHCII+ Ly6Chi monocytes in the bone marrow. All data represent mean ± SEM of one of 4 independent experiments, n = 4. * denotes p<0.05.

Fig 8

doi: https://doi.org/10.1371/journal.ppat.1006616.g008