Convergent evolution involving dimeric and trimeric dUTPases in pathogenicity island mobilization
Native-Page gels were performed to analyze the effect of the substrate and product on the interaction of two inducing dimeric Duts, (A) ϕDI and (B) ϕO11, with Stl. Increasing concentrations (from 0 to 1000 μM) of dUPNPP (top gels) or dUMP (down gels) were added to equimolecular concentrations of each Dut with Stl, and the Dut-Stl complex formation was evaluated by Native-PAGE. Notice the reduction of the Stl-Dut complex band when the dUTP analogue but not the dUMP product was present. For ϕDI a new band (labeled with a red asterisk) corresponding to the complex between dUPNPP and the Dut can be observed when the Stl-Dut complex disappears. For each experiment a representative gel of 3 independents assays is shown.