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Botulinum neurotoxin C mutants reveal different effects of syntaxin or SNAP-25 proteolysis on neuromuscular transmission

Fig 7

Imaging of cleaved SNAP-25 and syntaxin-1 in muscles treated with BoNT/C α-3W.

Soleus muscles of mice treated with BoNT/C α-3W and used for the analysis of EJPs of Fig 5B were fixed immediately after the electrophysiological recordings and stained for (A) cleaved SNAP-25 (SNAP-25c) or (B) Syntaxin-1A/1B (Stx-1A/1B), both shown in red. NMJs were spotted with α-Bungarotoxin (α-BTX, in green). The first row of panels represents the staining of a control muscle. Scale bar, 10 μm.

Fig 7

doi: https://doi.org/10.1371/journal.ppat.1006567.g007