Botulinum neurotoxin C mutants reveal different effects of syntaxin or SNAP-25 proteolysis on neuromuscular transmission
Soleus muscles of mice treated with BoNT/C-wt and used for the analysis of EJPs of Fig 5B were fixed immediately after the electrophysiological recordings and stained for (A) cleaved SNAP-25 (SNAP-25c) or (B) Syntaxin-1A/1B (Stx-1A/1B), both shown in red. NMJs were spotted with α-Bungarotoxin (α-BTX, in green). The first row of panels represents the staining of a control muscle. Scale bar, 10 μm.