Botulinum neurotoxin C mutants reveal different effects of syntaxin or SNAP-25 proteolysis on neuromuscular transmission
CGNs were treated with the indicated concentrations of BoNT/C-wt or mutants for 12 hours. Neurons were then fixed and stained with an antibody against cleaved SNAP-25 (SNAP-25c, in red) and neurofilament-200 (NF200, in green). Neurodegeneration was evaluated following the appearance of varicosities along neurites and the loss of NF200 staining. Images are representative of at least three independent experiments. Scale bar, 10 μm.