Destabilizing polymorphism in cervid prion protein hydrophobic core determines prion conformation and conversion efficiency
Resistance to PK was assessed using different PK concentrations to digest mWTD-wt (left panel) and -116AG (right panel) brain homogenate. (a) The PrPres Western blot pattern was revealed using monoclonal antibody 4H11 and (b) assessed by densitometric analysis of PrPres signals. *P <0.05 and ** P <0.01 refers to differences between PK resistance of mWTD-wt (blue bars) and -116AG (red bars) and evaluated using unpaired student’s t-test (GraphPad Prism software). (c) Conformational stability of mWTD-wt (blue line) and -116AG (red line) was assessed by densitometric analysis of PrPres signal after guanidine denaturation. *P <0.05 refers to differences between [GdnHCl1/2] from best-fitted sigmoid curves evaluated using unpaired student’s t-test (GraphPad Prism software).