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Destabilizing polymorphism in cervid prion protein hydrophobic core determines prion conformation and conversion efficiency

Fig 1

Biochemical properties of WTD isolates.

Resistance to PK was assessed using different PK concentrations to digest wt (left panel) and 116AG (right panel) brain homogenates. (a) The PrPres Western blot pattern was revealed using monoclonal antibody 4H11 and (b) assessed by densitometric analysis of PrPres signals. *P <0.05 refers to differences between PK resistance of wt (blue bars) and 116AG (red bars) and evaluated using unpaired student’s t-test (GraphPad Prism software). (c) Conformational stability of wt (blue line) and 116AG (red line) assessed by densitometric analysis of PrPres signals after guanidine denaturation followed by PK digestion. *P <0.05 refers to differences between [GdnHCl1/2] from best-fitted sigmoid curves evaluated using unpaired student’s t-test (GraphPad Prism software).

Fig 1

doi: https://doi.org/10.1371/journal.ppat.1006553.g001