Tissue-specific control of latent CMV reactivation by regulatory T cells
Fig 1
Activated Treg are increased in the spleen during latent MCMV.
Splenocytes were isolated from naïve (9.5months old, white bars), or aged matched MCMV-latently infected mice (black bars, 8 months post- inoculation with1× 106 pfu of MCMV). Cells were stained for CD4, CD69, and Foxp3 and analyzed by flow cytometry. Plots (A) and bar graph (B) show the frequency of Foxp3+ cells in total CD4+ cells from naïve and MCMV infected mice as described above (mean+SEM). Representative histograms (C) and bar graph (D) show the frequency of CD69+ on gated Foxp3+ cells from naïve and MCMV infected mice as described above (mean+SEM). Data is representative of two independent experiments. Naïve (N = 4), WT MCMV infected (N = 6). Statistical analysis (**p ≤ 0.01, Student’s t-test).