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Opposing roles of Toll-like receptor and cytosolic DNA-STING signaling pathways for Staphylococcus aureus cutaneous host defense

Fig 2

Toll-like receptor and STING signaling dominate the transcriptional response to S. aureus in murine macrophages.

(A) RNA-Seq of Myd88/Trif-/- macrophages was compared to that of WT macrophages. Shown is the % expression value of the 369 genes induced by live S. aureus in Myd88/Trif-/- macrophages compared to WT macrophages. The average expression from two separate experiments of WT and Myd88/Trif-/- macrophages treated at the same time with the same dose of S. aureus at 5 different time points in each experiment is shown. (B) Known, highly significant Jaspar 2016 motif enrichment of genes separated by their degree of dependence on Myd88/Trif-/- following S. aureus infection. (C) RNA-Seq of StingGt/Gt macrophages was compared to that of WT macrophages. Shown is the % expression value of the 369 genes induced by live S. aureus in StingGt/Gt macrophages compared to WT macrophages. The average expression from two separate experiments of WT and StingGt/Gt macrophages treated at the same time with the same dose of S. aureus at 5 different time points in each experiment is shown. (D) Known, highly significant Jaspar 2016 motif enrichment of genes separated by their degree of dependence on STING following S. aureus infection.

Fig 2

doi: https://doi.org/10.1371/journal.ppat.1006496.g002