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Opposing roles of Toll-like receptor and cytosolic DNA-STING signaling pathways for Staphylococcus aureus cutaneous host defense

Fig 1

Kinetic properties of the transcriptional cascade to live and HK S. aureus.

(A) Activation kinetics are shown for inducible genes from BMDMs stimulated with an MOI of 10 of S. aureus at indicated time points. Shades of blue to red indicate percentile values from low to high expression. Shown are all genes meeting RPKM >1, Fold change >5, and p<0.05 by DESeq. The average of at least 4 separate experiments is shown for each time point (B) The average fold of transcript levels of inducible genes separated within representative clusters by time point the genes reach their maximal expression level are shown. (C) Known, highly significant Jaspar 2016 motif enrichment of genes which reach maximal expression levels at the indicated time points following S. aureus infection. (D) Activation kinetics are shown for inducible genes from BMDMs stimulated with the equivalent of an MOI of 10 HK S. aureus at indicated time points. Shades of blue to red indicate percentile values from low to high expression. Induction and expression criteria are as in A. Shown are all genes meeting RPKM >1, Fold change >5, and p<0.05 by DESeq. The average of at least 2 separate experiments is shown for each time point (E) The average fold of transcript levels of inducible genes separated within representative clusters by time point the genes reach their maximal expression level are shown. (F) Known, highly significant Jaspar 2016 motif enrichment of genes which reach maximal expression levels at the indicated time points following S. aureus infection. (G) Venn diagram showing degree of overlap between all genes which meet expression and induction threshold criteria in macrophages in response to live or HK S. aureus treatment.

Fig 1

doi: https://doi.org/10.1371/journal.ppat.1006496.g001