A virulence-associated filamentous bacteriophage of Neisseria meningitidis increases host-cell colonisation
A. Immunofluorescence microscopy performed on the phage preparation using the polyclonal antibody anti-MDAORF4 directed against the major capsid protein of MDA. B. Z5463gfp and Z5463gfpΔMDA strains were grown on FaDu epithelial cells for 18 hours under constant flow. In one well, MDAΦ was added to the growth of the prophage deleted strain during the entire experiment at a final concentration of 1012 particles/mL. At least three independent experiments were performed. The results are normalized as a percentage of the mean biomass of the wild-type strain, which was set to 100%. Error bars indicate the standard errors of the mean (SEM). **p < 0.001 (One-way ANOVA), NS: not significant p value.