Negative modulation of suppressive HIV-specific regulatory T cells by IL-2 adjuvanted therapeutic vaccine
(A) Gating strategy used for detecting CMV-specific CD4+CD25+CD134+ CD39+FoxP3+ Tregs, and IFN-γ producing CD25+CD134+CD39-FoxP3- and CD25+CD134+ CD39-FoxP3+. PBMCs from CMV+ individuals were stimulated with CMV lysate for 44hrs. Flow cytometry was used to detect CMV-specific cells as described in the methods. (B) Mean fluorescence intensity (MFI) of Helios, CD15s, CTLA-4, ICOS, PD-1 and T-bet molecules were measured by flow cytometry in 8 CMV+ individuals to detect CMV-specific subsets (CD134+CD25+Foxp3+CD39+, CD134+CD25+Foxp3+CD39- and CD134+CD25+Foxp3-CD39-). P values were considered significant when < 0.05.