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Negative modulation of suppressive HIV-specific regulatory T cells by IL-2 adjuvanted therapeutic vaccine

Fig 5

HIV-specific CD4+ T-cell responses were amplified by IL-2 treatment and inversely correlate with VL after treatment interruption.

(A) Example of gating strategy used for detecting antigen-specific CD4+CD25+CD134+ cells after in vitro stimulation with p24 or none (OX40 assay), at wk0, wk16 and wk36. (B and D) Representation of HIV- and CMV- specific CD4+CD25+CD134+ percentages in patients at wk0, wk16 and wk36 using the gating strategy shown in (A). (C and E) Correlations between %CD4+CD25+CD134+ and viral load at resuming ART, at wk36 for p24 and CMV, respectively. Correlations were calculated using spearman correlation coefficients (Prism 5.0, version 5.0d). P values were considered significant when < 0.05.

Fig 5

doi: https://doi.org/10.1371/journal.ppat.1006489.g005