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Negative modulation of suppressive HIV-specific regulatory T cells by IL-2 adjuvanted therapeutic vaccine

Fig 3

Subcutaneous injections of IL-2 decrease PD-1 expression in CD4+ and CD8+ T cells.

(A) Gating strategy for CD4+ HLA-DR+CD38+ and CD8+ HLA-DR+CD38+ cell frequencies measured at wk0, wk16 and wk36. Cells were first gated on CD3+CD4+ before depicting CD38+HLA-DR+ subset. (B) Gating strategy for CD4+CD95+PD-1+ and CD8+CD95+PD-1+ cell frequencies that were measured at wk0, wk16 and wk36. CD3+CD4+ cells were first selected, then CD95+ cell gate applied and finally PD-1+ cells were depicted. Bottom panel shows Mean Fluorescence Intensity (MFI) for PD-1 on CD95+CD4+ and CD95+CD8+. Prism 5.0, version 5.0d, (GraphPad Software, Inc.) was used for statistical analyses. P values were considered significant when < 0.05.

Fig 3

doi: https://doi.org/10.1371/journal.ppat.1006489.g003