Intestinal calcium and bile salts facilitate germination of Clostridium difficile spores
Tc binds to CspC (A) facilitating movement of glycine or calcium through the spore coat and outer membrane (B). Glycine then interacts with an unknown receptor (C) inducing the release of Ca2+ from the spore core (D). Ca2+ from the environment or the spore core activates CspB (E), which processes pro-SleC (F) subsequently initiating cortex hydrolysis (G). This leads to full core rehydration (H), complete release of DPA (I) and spore outgrowth.