Intestinal calcium and bile salts facilitate germination of Clostridium difficile spores
Cd630, ΔgerS, ΔcspB, ΔcspC, and ΔsleC spores were incubated with 0.2% Tc and with (+) or without (-) the indicated co-cogerminant: 50 mM glycine (a) or 60 mM CaCl2 (b). Germination was measured by loss of OD600 at 37°C over the course of one hour. (a-b). Western blot of SleC from WT, ΔgerS, ΔcspB, or ΔcspC spores incubated at 37°C for 15 minutes with 1% Tc and the indicated concentrations of glycine (c) or CaCl2 (d). Germination assays were performed in triplicate. Germination assays and western blots are representative of 3 independent spore preps. Error bars are mean plus or minus SD.