Streptococcus gallolyticus subsp. gallolyticus promotes colorectal tumor development
Approximately 1x105 cells/well were incubated with bacteria (~105 cfu/well) or media only for 12 hours in a 6 well plate. Whole cell or nuclear lysates as well as RNA were extracted and analyzed by western blot assays using specific antibodies (A-F, H, and I) or RT-qPCR (G). Representative images are shown (A, C, E and H). A and B, HCT116; C and D, HT29; E and F, FHC. Band intensity was quantified using Image J, normalized to β-actin or lamin B first and then normalized to the cells only control. G. Sg increased the expression of cyclin D1 in HCT116 and HT29 cells. ΔΔCT was first normalized to GAPDH then to cells cultured in media only. Results in panels B, D, F, G and I were combined from at least 3 experiments. Data are presented as mean ± SEM. Data were analyzed using one-way, two-tailed ANOVA followed by SNK test. *, p < 0.05; **, p < 0.01.