The transcriptome of HIV-1 infected intestinal CD4+ T cells exposed to enteric bacteria
Fig 9
HIV-1 infection differentially alters multiple pathways in microbe-exposed gut CD4+ T cells.
Prevotella stercorea were utilized as representative enteric bacteria found in the mucosa of HIV-1-infected individuals. (A) Overlap in HIV-1-altered genes between gut CD4+ T cells exposed or not exposed to bacteria. (B) Comparison of HIV-1-altered pathways with or without microbial exposure using Ingenuity Pathway Analyses (IPA). (C) Comparison of HIV-1-altered upstream regulators based on IPA. (D) HIV-1-altered genes in microbe-exposed CD4+ T cells. Colored bars were discussed in the text. (E) Induction of Granzyme B in HIV-1-infected, microbe-exposed gut CD4+ T cells. The percentages of granzyme B+ cells were evaluated in CD3+CD8- cells from mock-infected LPMCs exposed to Prevotella stercorea versus HIV-1-eGFP+ cells from HIV-1-infected LPMCs exposed to Prevotella stercorea. Data were analyzed using a paired 2-tailed Student’s t-test. (F) IPA-predicted downstream effects, based on differentially altered genes.