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A Superoxide Dismutase Capable of Functioning with Iron or Manganese Promotes the Resistance of Staphylococcus aureus to Calprotectin and Nutritional Immunity

Fig 4

SodM can be metallated with either Mn or Fe in S. aureus.

The S. aureus ΔsodAΔsodM mutant expressing SodM from a plasmid was grown in NRPMI supplemented with either 1 μM FeCl2 or 1 μM MnCl2 and SOD activity was assessed in cell lysates (24.8 μg of total protein), with and without peroxide treatment. Peroxide treatment was used to inactivate Fe-containing SODs. Purified SodM (0.3 μg), loaded with either Mn or Fe, were included as controls. The experiment was repeated 3 times, and representative gels are shown.

Fig 4

doi: https://doi.org/10.1371/journal.ppat.1006125.g004